Journal: Nature Communications

Article Title: Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation

doi: 10.1038/s41467-024-49380-0

Figure Lengend Snippet: a Experimental approach to determine clonal composition of AVE nests. b Expression of clonal labels (red, cyan) and Cer1:H2B-Venus reporter (yellow) in cultures differentiated as in a . Insets on the right show examples of Cer1:H2B-Venus-expressing nests with a single clonal label (top, 13/30 nests), or with multiple labels (bottom, 17/30 nests). One out of n = 2 independent experiments shown. c Immunostaining for OTX2 (magenta) and H2B-Venus (yellow) of Cer1:H2B-Venus reporter cells differentiated for 3 days after an extended doxycycline pulse with 50 ng/ml ActivinA (AA), together with 3 µM Chir99021 (Chi), 20 µM XAV939 (XAV), or 2 µM IWP2 as indicated. One out of n = 3 independent experiments shown. d Flow cytometry of cells differentiated and stained as in c . e Mean percentage of Cer1:H2B-Venus; OTX2 double-positive cells differentiated as in c . n = 4 independent experiments, error bars indicate SD. ** p < 0.001 for AA vs. AA + Chi and AA vs. AA + XAV, and p = 0.0045 for AA vs. AA + IWP2 (two-tailed, unpaired t -test). f Same as e but showing percentage of OTX2-positive cells. ** p < 0.001 for AA vs. AA + Chi and p = 0.0001 for AA vs. AA + XAV, * p = 0.0434 for AA vs. AA + IWP2 (two-tailed, unpaired t -test). Scale bars: 200 µm (( b ) overview); 20 µm (( b ) inset); 500 µm (( c ) overview); 50 µm (( c ) inset). Source data for e , f are provided in the Source Data file.

Article Snippet: To test the functionality of the putative AVE, we adopted a protocol to trigger mesoderm differentiation in ESC aggregates , consisting of a 24 hour treatment with 3 μM Chir99021 (Chi) on day 2, followed by analysis 24 hours after the end of the Chi pulse (Supplementary Fig. ).

Techniques: Expressing, Immunostaining, Flow Cytometry, Staining, Two Tailed Test

Journal: Hypertension Research

Article Title: Aktiia cuffless blood pressure monitor yields equivalent daytime blood pressure measurements compared to a 24-h ambulatory blood pressure monitor: Preliminary results from a prospective single-center study

doi: 10.1038/s41440-023-01258-2

Figure Lengend Snippet: Illustration of Aktiia monitor. A Aktiia bracelet with embedded optical sensors. B Representation of the experiment setup with Aktiia bracelet on patient’s wrist (green) and the ABPM device (red). C Aktiia application displays BP measurements on a smartphone

Article Snippet: The patients used the Aktiia monitor during the 12 weeks of the CR program, while 24-hour ABPM (Dyasis 3, Novacor, Paris, France) was performed twice – on the first and last day of the CR program.

Techniques:

Journal: Hypertension Research

Article Title: Aktiia cuffless blood pressure monitor yields equivalent daytime blood pressure measurements compared to a 24-h ambulatory blood pressure monitor: Preliminary results from a prospective single-center study

doi: 10.1038/s41440-023-01258-2

Figure Lengend Snippet: Scatter plots (upper) and Bland-Altman plots (bottom) comparing daytime BP measurements performed by Aktiia monitor (7-day average) and ABPM (1-day average). A . Comparison of daytime DBP. B . Comparison of daytime SBP. C . Comparison of daytime HR. Pearson’s correlation ( R 2 ) between the two modalities is shown on the bottom right of the scatter plots. ROI within ±10 mmHg and ±15 mmHg are highlighted in dark grey and light grey, respectively. The percentage of agreement for each region is shown on the bottom left of the Bland-Altman plots. The solid black line denotes the average mean of differences, while the dotted lines denote the limits of agreement (±1.96 σ ). BPM – beats per minute. The dark green dots represent first week sessions (35 sessions), and the light green dots represent last week sessions (15 sessions)

Article Snippet: The patients used the Aktiia monitor during the 12 weeks of the CR program, while 24-hour ABPM (Dyasis 3, Novacor, Paris, France) was performed twice – on the first and last day of the CR program.

Techniques: Comparison